Bisphenol-A publicity alters liver, kidney, and pancreatic Klotho expression by HSP60-activated mTOR/autophagy pathway.
PMID:
Cell Mol Biol (Noisy-le-grand). 2023 Jul 31 ;69(7):109-117. Epub 2023 Jul 31. PMID: 37715411
Summary Title:
Bisphenol-A publicity alters liver, kidney, and pancreatic Klotho expression by HSP60-activated mTOR/autophagy pathway in male albino rats.
Summary:
The impact of bisphenol-A (BPA) on Klotho protein (aging-suppressing protein) expression in numerous physique organs has not been sufficiently addressed by literature research. The examine investigated the impression of BPA on Klotho expression in a number of organs together with the liver, kidney, and pancreas and advised the concerned molecular pathways. Twenty-seven male Wistar albino rats have been divided into 3 equal teams: management, low-dose BPA (4.5µg/L), and high-dose BPA (8µg/L) teams in ingesting water for 45 consecutive days. Liver, kidney, and pancreatic specimens have been ready for a gene examine of Klotho, HSP60, mTOR, and ULK1 mRNA expressions. Additionally, the tissue specimens have been measured for malondialdehyde (MDA), superoxide dismutase (SOD), and nitric oxide (NO) ranges. Paraffin-embedded sections have been additionally ready and subjected to Hematoxylin and Eosin (H&E) staining and immunohistochemical detection of Klotho and HSP60. The outcomes revealed an alteration within the MDA, SOD, NO tissue ranges, disturbed gene expression profile, and apoptotic modifications within the histological findings of the examined organs which have been apparent (p
