Entrance Pharmacol. 2023 ;14:1228722. Epub 2023 Jul 4. PMID: 37469873
Berberine enhances the operate of db/db mice isletβcell by way of GLP-1/GLP-1R/PKA signaling pathway in intestinal L cell and isletαcell.
The proof on berberine stimulating the secretion of GLP-1 in intestinal L cell has been studied. Nevertheless, few analysis has explored its function on producing GLP-1 of isletαcell. Our experiment goals to make clear the mechanism of berberine selling the secretion of GLP-1 in intestinal L cell and isletαcell, activating GLP-1R and its downstream molecules by way of endocrine and paracrine methods, thus bettering the operate of isletβcell and treating T2DM.After confirming that berberine can decrease blood glucose and enhance insulin resistance in db/db mice, the id upkeep, proliferation and apoptosis of islet cells have been detected by immunohistochemistry and immunofluorescence. Then, the activation of berberine on GLP-1/GLP-1R/PKA signaling pathway was evaluated by Elisa, Western blot and PCR. Lastly, this mechanism was verified byexperiments on Min6 cells, STC-1 cells and aTC1/6 cells.Berberine ameliorates glucose metabolism in db/db mice. Moreover, it additionally will increase the quantity and enhances the operate of isletβcell. This course of is intently associated to enhance the secretion of intestinal L cell and isletαcell, activate GLP-1R/PKA signaling pathway by way of autocrine and paracrine, and improve the expression of its associated molecule similar to GLP-1, GLP-1R, PC1/3, PC2, PKA, Pdx1., the phenomenon that berberine enhanced the GLP-1/GLP-1R/PKA sign pathway had additionally been noticed, which confirmed the outcomes of animal experiments.Berberine can keep the id and regular operate of isletβcell, and its mechanism is said to the activation of GLP-1/GLP-1R/PKA sign pathway in intestinal L cell and isletαcell.