J Pharm Biomed Anal. 2023 Sep 20 ;234:115555. Epub 2023 Jul 6. PMID: 37473506
Results of (20 R)-Panaxadiol on NAFLD utilizing non‑focused metabolomics in stool.
Non-alcoholic fatty liver illness (NAFLD) is a scientific syndrome characterised by hepatocyte steatosis and adipose accumulation with the principle lesion within the hepatic lobule, however with no historical past of extreme alcohol consumption. NAFLD ranges from nonalcoholic fatty liver (NAFL) to nonalcoholic steatohepatitis (NASH), and will additional accumulate fibrosis resulting in cirrhosis. Many research have discovered that ginseng can deal with NAFLD. (20 R)-Panaxadiol (PD) is a panax ginseng diol kind compound, has been proved that may deal with the weight problems. This examine needs to research the impact of PD on non-alcoholic liver illness. We used 20 ob/ob mice and 10 C57BL/6 J mice. C57BL/6 J mice as CONTROL group, ob/ob mice had been divided into mannequin group and PD group. In PD group, ob/ob mice had been handled with PD for eight weeks(10 mg/kg, the CON and OB group was given the identical quantity of sodium carboxymethyl cellulose), detected the load, meals consumption and serum index, noticed the HE staining of liver and gut, carried out the 16 S rRNA and untargeted metabolomics evaluation used mice feces, and confirm the outcomes by detect the expression of TNF-α, MDA and SOD. In vivo outcomes, PD can enhance irregular glucose and lipid metabolism and liver perform. In 16 S rRNA outcome, we discovered helpful micro organism Muribaculaceae and Lactobacillus elevated; in untargeted metabolomics evaluation, inflammatory metabolites prostaglandin (PG) and lipopolysaccharide (LPS) decreased, antioxidant metabolites FAD and lipoic acid elevated. Then, we proceeded the affiliation evaluation of intestine microbiota and metabolites, the outcome confirmed intestine microbiota have strongly related to anti-inflammatory and antioxidant metabolites. As well as, PD improves intestinal wall integrity. In the meantime, the expression of TNF-α、MDA and SOD had been detected, it was verified that PD has the impact of antioxidant and anti-inflammation. Our examine confirmed that PD, as an energetic ingredient of ginseng, can play an anti-inflammatory and antioxidant function by enhancing intestinal metabolites, thereby stopping and treating non-alcoholic fatty liver illness to a sure extent.