Results of ginkgolide B in opposition to harm of cultured hippocampal neurons brought on by glutamate.
PMID: Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2007 Might;23(2):155-8. PMID: 21179757 Summary Title:
[Effects of ginkgolide B against damage of cultured hippocampal neurons caused by glutamate].
Summary:
AIM: To analyze protecting results of ginkgolide B (GB) in numerous administration modes on glutamate-induced neuronal harm.METHODS: Important GB had been obtained by supercritical CO2 fluid extraction. Glutamate excitotoxicity had been examined in main cultures from neonatal Wistar rat, by utilizing of Trypan blue dye staining, testing the lactate dehydrogenase leakage from cultured neurons and terminal deoxynucleotidyl transferase-mediated nick finish labeling (TUNEL) methodology. The protecting results of GB in numerous administration modes (pre-treatment and post-treatment) had been adopted and in contrast with the NMDA receptor uncompetitive antagonist-MK-801 in acute-treatment.RESULTS: Therapy with GB in two administration modes each might enhance ratio of surviving neuron, lower LDH efflux and cut back ratio of neuron apoptosis in numerous diploma, trusted dose in sure vary. The protecting impact of pre-treatment was superior to post-treatment, however inferior to MK-801.CONCLUSION: GB can shield neurons in opposition to glutamate harm, and preventive utilizing has extra effectivity. The potential mechanism of its neural safety could also be not solely associated to PAF receptor. If the predominant safety impact of GB in pretreatment is taken into account, precautionary intervention to high-risk inhabitants might have extra worth.
