Results of ginkgolide B on proliferation, phagocytosis, NO and ROS manufacturing of murine peritoneal macrophages.
PMID: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Jan ;28(1):4-7. PMID: 22230494 Summary Title:
[Effects of ginkgolide B on proliferation, phagocytosis, NO and ROS production of murine peritoneal macrophages in vitro].
Summary:
AIM: To discover the potential immunomodulatory results and associated mechanisms of ginkgolide B (GB), a recognized potent antagonist of platelet-activating issue receptor, we investigated the proliferation, phagocytosis, NO and ROS manufacturing of macrophage.METHODS: After murine peritoneal macrophages (PMs) preparation, PMs have been handled with completely different concentrations of GB earlier than tradition time after which activated by LPS. Drug toxicology and PM proliferation have been measured by MTT assays. Fluorescent beads ingestion and stream cytometry have been used to evaluate phagocytosis of LPS-activated PMs. Griess reagent system was used to find out the quantity of LPS-induced NO manufacturing. H2DCFDA labeling and stream cytometry have been used to hint ROS degree of each relaxation and LPS-activated PMs.RESULTS: In a dose-dependent method, GB (5, 10, and 20μmol/L) considerably suppressed the phagocytosis in addition to NO and ROS manufacturing at 24 h and inhibited cell proliferation at 48 h after LPS stimulation.CONCLUSION: Based on these attention-grabbing results of GB on macrophage behaving and functioning, it is fairly cheap to do additional research of GB as a nature occurring immunomodulator candidate.
