Ginkgolides and Ginkgo flavonoids would possibly defend towards apoptosis of hippocampal neurons.
PMID:
Heliyon. 2015 Sep ;1(1):e00020. Epub 2015 Sep 21. PMID: 27441214
Summary Title:
The protecting mechanism of Ginkgolides and Ginkgo flavonoids on the TNF-αinduced apoptosis of rat hippocampal neurons and its mechanisms in vitro.
Summary:
OBJECTIVE: To discover the neuroprotective mechanism of Ginkgolides or Ginkgo flavonoids on the TNF-αinduced apoptosis of cultured rat hippocampal neurons.MATERIALS AND METHODS: Main hippocampal neurons had been remoted from rat brains and cultured with (mannequin group) or with out (management group) addition of tumor necrosis factor-α(TNF-α, remaining focus of 40 ng/ml) to induce apoptosis. TNF-αinduced cultures had been divided into mannequin group, Ginkgolides pre-treatment group (20 μg/ml) and Ginkgo flavonoids pre-treatment group (12 μg/ml). CCK8 was used to evaluate cell viability whereas Hoechst 33258 staining, Circulation cytometry and TUNEL kits had been all employed to find out apoptotic neurons. Expression ranges of Bcl-2, Bax, Caspase-3, Caspase-7, Caspase-8, Caspase-9 and Cytc had been estimated by qRT-PCR.RESULTS: Cell viability was considerably improved in Ginkgolides pre-treatment group or Ginkgo flavonoids pre-treatment group in contrast with that in mannequin group. Apoptotic neurons had been considerably much less in Ginkgolides pre-treatment group or Ginkgo flavonoids pre-treatment group than these in mannequin group. Transcription ranges of caspase-7, caspase-8, caspase-3, caspase-9, Bax and Cytc had been down-regulated, whereas transcription ranges of Bcl-2 was up-regulated in Ginkgolides pre-treatment or Ginkgo flavonoids pre-treatment group than these in mannequin group.CONCLUSIONS: Ginkgolides and Ginkgo flavonoids would possibly defend towards apoptosis of hippocampal neurons by means of inhibiting loss of life receptor pathway or mitochondrial pathway underneath TNF-αbackground.
