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Ginkgolides induced ischemic tolerance and its doable molecular mechanism.

PMID: Zhonghua Yi Xue Za Zhi. 2007 Sep 11;87(34):2432-5. PMID: 18036326 Summary Title:

[Ginkgolides induced ischemic tolerance and its possible molecular mechanism: experiment with rat pheochromocytoma cell line PC12].


OBJECTIVE: To discover the ischemic tolerance induced by Ginkgolides in PC12 cells and its doable molecular mechanism.METHODS: An ischemic mannequin was developed in PC12 cell line with deprivation of oxygen-glucose (OGD). PC12 cells was randomly divided into 4 teams: 9 hours ischemia group, 1.5 hours ischemic preconditioning + 9 hours ischemia group, Ginkgolides preconditioning + 9 hours ischemia group and management group. Cells viability was examined by MTT assay and mobile morphology was analyzed below the phase-contrast microscope. The molecular mechanism of Ginkgolides induced ischemic tolerance was pinpointedby analyzing the expression of hypoxia-inducible factor-1 alpha (HIF-1alpha) and erythropoietin (EPO). The DNA binding actions of HIF-1 in PC12 cells had been examined by electrophoretic mobility shift assay.RESULTS: In ischemic mannequin, the viability of PC12 cells was decreased (49.3 +/- 2.8)% after OGD for 9 hours. Nevertheless, Ginkgolides pretreatment may remarkably improve the viability of PC12 cells (65.9 +/- 2.8)% (P

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