PMID:
J Cell Biochem. 2008 Feb 1 ;103(2):564-75. PMID: 17647269
Summary Title:
Ginkgolides shield PC12 cells towards hypoxia-induced damage by p42/p44 MAPK pathway-dependent upregulation of HIF-1alpha expression and HIF-1DNA-binding exercise.
Summary:
We hypothesized that the neuroprotective position of the standardized Ginkgo biloba (Ginkgoaceae) extract EGb 761 underneath hypoxic situations is likely to be related to its perform to extend HIF-1 exercise based mostly on the truth that oxygen availability is essential for mobile metabolism and viability and that HIF-1 performs a necessary position in mobile oxygen homeostasis underneath hypoxic situations. On this research, we due to this fact investigated the consequences of ginkgolides, the primary constituent of the non-flavone fraction of EGb 761, on the content material and exercise of HIF-1alpha, a key issue to find out HIF-1 exercise, in hypoxic PC12 cells induced by cobalt chloride. Our knowledge demonstrated that ginkgolides have a major protecting position towards hypoxia-induced damage within the PC12 cells. The findings additionally strongly help our speculation that the protecting position of ginkgolides is as a result of up-regulation of HIF-1alpha protein expression and modification by means of the ginkgolides-induced activation of the p42/p44 MAPK pathway. As well as, it was evident that ginkgolides may considerably improve the HIF-1 DNA binding exercise, which could even be related to the protecting results of ginkgolides by selling the expression of goal genes of HIF-1 underneath hypoxic situations.
