PMID:
Sci Rep. 2022 Oct 12 ;12(1):17101. Epub 2022 Oct 12. PMID: 36224327
Summary Title:
In vitro inhibition of SARS-CoV-2 An infection by dry algae powders.
Summary:
Chlorella spp., Spirulina spp., and fucoidan dry powders, are commercialized as meals dietary supplements and are thought-about protected for human consumption. Their broad-spectrum antiviral properties have been studied, nonetheless, their impact towards SARS-CoV-2 stays unknown. We investigated the potential antiviral exercise of three algae powders: Chlorella vulgaris, Arthrospira maxima (Spirulina) and fucoidan purified from marine brown algae Sargassum spp. towards SARS-CoV-2 an infection in vitro. Vero cells have been incubated with 70 μg/ml of every algae powder and both 50 or 100 TCID/ml of SARS-CoV-2, in two kinds of experiments (pretreatment and simultaneous) and evaluating two sorts of solvents (DMEM and DMSO). Chlorella vulgaris powder, inhibited SARS-CoV-2 an infection in all assays; viral RNA was considerably diminished in supernatants at 24, 48, 72, and 96 h post-infection, the best distinction in viral load (8000-fold) was noticed after 96 h. Arthrospira maxima powder inhibited SARS-CoV-2 an infection utilizing 50 TCID/ml for each experimental schemes, however safety % was decrease when viral inoculum was enhance to 100 TCID/ml; viral RNA decreased 48 h after an infection, reaching a 250-fold distinction at 72 h. Fucoidan powder partially inhibited SARS-CoV-2 an infection since no CPE was noticed in 62.5% of trated cultures in DMEM, however the antiviral exercise was elevated to 100% of safety when DMSO was used as solvent. All of the algae samples confirmed excessive antiviral exercise towards SARS-CoV-2 with a SI above of 18. These outcomes counsel that every one three algae samples are potential therapeutic candidates for the remedy of COVID-19.
