These outcomes point out the potentials of PUFA-rich extracts from Chlorella sp. S14 to scale back viability and modulate redox standing in A549 and MCF-7 cells.
PMID:
Molecules. 2021 Jul 6 ;26(14). Epub 2021 Jul 6. PMID: 34299383
Summary Title:
Chemical Characterization, Antiproliferative and Antioxidant Actions of Polyunsaturated Fatty Acid-Wealthy Extracts fromsp. S14.
Summary:
Microalgae is a wealthy supply of polyunsaturated fatty acid. This examine was performed to establish and isolate microalgal pressure with the potentials for producing polyunsaturated fatty acids (PUFAs) and decide its cytotoxic impact on some most cancers cells. The algal pressure (sp. S14) was cultivated utilizing modified BG-11 media, and algal biomass obtained was used for fatty acid extraction. Gasoline chromatographic-mass spectrometry was used to establish and quantify the degrees of the fatty acid constituents. The full content material of monounsaturated fatty acids (1.12%) was low in comparison with polyunsaturated fatty acids (PUFAs) (52.87%). Moreover, n-3 PUFAs accounted for (12.37%) of complete PUFAs with the presence ofα-linolenic acid (2.16%) and-11,14,17-eicosatrienoic acid (2.16%). The PUFA-rich extract didn’t exhibit a cytotoxic impact on regular cells. Therapy with the PUFA-rich extract (150µg/mL) considerably lowered cell viability in MCF-7 (31.58%) and A549 (62.56%) cells after the 48 h therapy. Moreover, therapy of MCF-7 with fatty acid extracts (125 and 150µg/mL) confirmed a big discount in MDA ranges, improve in catalase actions and reduce in GSH stage in comparison with untreated cells. Nonetheless, a slight lower in MDA stage was noticed in A549 cells after the 48 h therapy. There aren’t any vital modifications in catalase actions and GSH stage in handled A549 cells. Nonetheless, a slight discount of NO ranges was noticed in handled MCF-7 and A549 cells. These outcomes point out the potentials of PUFA-rich extracts fromsp. S14 to scale back viability and modulate redox standing in A549 and MCF-7 cells.
