Gene. 2021 Could 5 ;779:145494. Epub 2021 Feb 13. PMID: 33588036
Transcriptome evaluation reveals doable antitumor mechanism of Chlorella exopolysaccharide.
Microalgae, one of the vital essential courses of biomass producers, can produce exopolysaccharides much like micro organism. The exopolysaccharide from Chlorella (CEPS) shows outstanding anticancer exercise the mechanism of which stays to be elucidated. On this research, we analyzed the inhibitory impact of CEPS on the expansion of HeLa cells. The outcomes confirmed that CEPS inhibited the proliferation, decreased the viability, and adjusted the morphology of HeLa cells. Transcriptome evaluation confirmed that 1894 genes have been differentially expressed within the CEPS-treated group in contrast with the management group, together with 1076 genes that have been upregulated and 818 genes that have been downregulated. The outcomes of gene operate enrichment evaluation confirmed that the differentially expressed genes (DEGs) have been considerably enriched in apoptosis and tumor-related organic processes and took part in a number of most cancers and apoptosisrelated signaling pathways, together with the MAPK signaling pathway, TNF signaling pathway, and the PI3K-Akt signaling pathway. The protein-protein interplay community recognized 13 DEGs together with PTPN11, RSAD2, ISG15, IFIT1, MX2, IFIT2, OASL, OAS1, JUN, OAS2, XAF1, ISG20, and IRF9 as hub genes. Our outcomes counsel that CEPS is a promising therapeutic drug for the follow-up interventional remedy of most cancers.